探索关节软骨钙化层的结构形态及其与软骨非钙化层和软骨下骨之间的界面连接方式。 方 法 组织库获取自愿捐献的人体正常股骨髁新鲜标本20 个，男10 个，女10 个；年龄17 ～ 45 岁。常规制备石蜡横、纵切片，番红O/ 固绿和冯库萨染色观察钙化层形态结构；扫描电镜观察软骨各层之间的界面连接方式；连续切片结合建模技术建立骨软骨三维模型。 结果 关节骨软骨复合组织番红O/ 固绿染色结果示软骨红染，软骨下骨蓝染，钙化层位于潮线与黏合线之间；冯库萨染色结果示钙化层黑染，结构及边界清晰，上界面以波浪状潮线结构与非钙化层紧密连接，下界面以凹凸不平的梳齿状结构与软骨下骨相互锚合；关节骨软骨剥离面及断面扫描电镜示钙化层与非钙化层以沟壑镶嵌方式相互嵌合；关节骨软骨复合组织纵切面观察，钙化层与软骨下骨间的黏合线呈凹凸不平的梳齿状结构；骨软骨三维模型观察结果与关节软骨自然剥离横断面扫描电镜观察结果基本一致。 结论 钙化层是关节软骨的重要结构，它通过特有界面连接方式将软骨牢牢固定在软骨下骨上。
To explore the shape and structure of calcified cartilage zone and its interface between the non-calcified articular cartilage and subchondral bone plate. Methods The normal human condyles of femur (n=20) were obtained from the tissue bank donated by the residents, 10 males and 10 females, aged 17-45 years. The longitudinal and transverse paraffin sections were prepared by the routine method. The shape and structure of calcified cartilage zone were observed with the
Safranin O/fast green and von kossa stain method. The interface conjunction among zones of cartilage was researched by SEM and the 3D structural model was establ ished by serial sections and model ing technique. Results Articular bone-cartilage safranin O/fast green staining showed that cartilage was stained red and subchondral bone was stained blue. The calcified cartilage zone was located between the tidemark and cement l ine. Von kossa staining showed that calcified cartilage zone was stained black and sharpness of structure border. Upper interface gomphosised tightly with the non-calcified cartilage by the wave shaped tidemark and lower interface anchored tightly with the subchondral bone by the uneven comb shaped cement l ine. The noncalcified cartilage zone was interlocked tightly in the manner of “ravine-engomphosis” by the calcified cartilage zone as observed under SEM, and the subchondral bone was anchored tightly in the manner of “comb-anchor” by the in the calcified cartilage zone 3D reconstruction model. Conclusion The calcified cartilage zone is an important structure in the articular cartilage. The articular cartilage is fixed firmly into subchondral bone plate by the distinctive conjunct interfaces of calcified cartilage zone.
引用本文： 王富友,杨柳,段小军,谭洪波,戴刚. 正常膝关节软骨钙化层形态结构研究. 中国修复重建外科杂志, 2008, 22(5): 524-527. doi: 复制