中国修复重建外科杂志

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用于周围神经再生的纤维基导电型复合支架制备及应用

戴伍飞 1,2 , 石嘉琪 1,2 Δ , 刘莎 1 , 徐梓淇 1,2 , 石逸瑾 1,2 , 赵亚红 2 , , 杨宇民 1 ,

1. 南通大学江苏省与教育部神经再生重点实验室 南通大学神经再生协同创新中心（江苏南通  226001） ;
2. 南通大学医学院（江苏南通  226001）

收稿日期: 2018-08-01;  修回日期:2019-01-05; 

基金项目: 国家自然科学基金重点项目（31830028）；国家自然科学基金重点研发计划项目（2017YFA0701304、2016YFC1101603）；国家自然科学基金面上项目（81671823、81701835）；南通大学大学生创新创业训练计划（201810304027Z）；江苏省政府海外留学奖学金（2018-2019）

通讯作者: 赵亚红, Email: zhaoyh108@ntu.edu.cn 杨宇民, Email: yangym@ntu.edu.cn

Δ共同第一作者

Preparation and application of fiber-based conductive composite scaffolds for peripheral nerve regeneration

DAI Wufei 1,2 , SHI Jiaqi 1,2 , LIU Sha 1 , XU Ziqi 1,2 , SHI Yijin 1,2 , ZHAO Yahong 2 , , YANG Yumin 1 ,

1. Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong Jiangsu, 226001, P.R.China ;
2. Nantong University School of Medicine, Nantong Jiangsu, 226001, P.R.China

Corresponding Author: ZHAO Yahong, Email: zhaoyh108@ntu.edu.cn YANG Yumin, Email: yangym@ntu.edu.cn

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目的 探讨一种“壳-芯”结构的聚吡咯/丝素蛋白（polypyrrole/silk fibroin，PPy/SF）纤维基导电型复合支架的制备方法、理化性能与生物相容性，为其用于组织工程神经研究奠定基础。 方法 采用 3D 打印结合原位聚合反应制备“壳-芯”结构导电纤维，联合静电纺形成 PPy/SF 纤维基导电型复合支架；另制备无 SF 内芯的 PPy 导电纤维及 SF 静电纺纤维。分别对各材料进行稳定性、生物力学、导电性、降解性能、生物活性检测，观察纤维基导电型复合支架的综合性能。 结果 与单纯无内芯的 PPy 导电纤维及 SF 静电纺纤维比较，“壳-芯”结构的纤维基导电型复合支架可以更好地保持稳定性能，增强复合支架的力学拉伸性，电导性维持长久，提升抗降解性能；同时 PPy/SF 纤维基导电型复合支架适合 NIH3T3 细胞附着，有利于细胞增殖，生物活性良好。 结论 PPy/SF 纤维基导电型复合支架满足人工神经移植物在导电性、稳定性、生物活性等方面的要求，为研制高性能、多功能的复合材料提供新思路。

Objective To explore the preparation method, physical and chemical properties, and biocompatibility of a conductive composite scaffold based on polypyrrole/silk fibroin (PPy/SF) fiber with " shell-core” structure, and to provide a preliminary research basis for the application in the field of tissue engineered neuroscience. Methods The conductive fibers with " shell-core” structure were prepared by three-dimensional printing combined with in-situ polymerization. PPy/SF fiber-based conductive composite scaffolds were formed by electrospinning. In addition, core-free PPy conductive fibers and SF electrospinning fibers were prepared. The stability, biomechanics, electrical conductivity, degradation performance, and biological activity of each material were tested to analyze the comprehensive properties of fiber-based conductive composite scaffolds. Results Compared with pure core-free PPy conductive fibers and SF electrospinning fibers, the PPy/SF fiber-based conductive composite scaffolds with " shell-core” structure can better maintain the stability performance, enhance the mechanical stretchability of the composite scaffolds, maintain long-term electrical activity, and improve the anti-degradation performance. At the same time, PPy/SF conductive composite scaffolds were suitable for NIH3T3 cells attachment, conducive to cell proliferation, and had good biological activity. Conclusion PPy/SF fiber-based conductive composite scaffolds meet the needs of conductivity, stability, and biological activity of artificial nerve grafts, and provide a new idea for the development of a new generation of high-performance and multi-functional composite materials.

关键词: 组织工程; 周围神经; 导电型; 导向性; 生物活性; 支架材料

Key words: Tissue engineering; peripheral nerve; conductive; orientation; bioactivity; scoffold material

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