中国修复重建外科杂志

中国修复重建外科杂志

新型前置式脂肪抽吸针对移植脂肪成活影响的实验研究

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目的探讨采用新型前置式脂肪抽吸针抽取脂肪对移植后脂肪成活的影响。方法选择 1 例行腹部脂肪抽吸术女性患者,其左、右侧腹部分别采用新型前置式脂肪抽吸针(实验组)和传统侧孔抽脂针(对照组)抽取脂肪。通过扫描电镜观察及葡萄糖转移实验,比较两组脂肪细胞体外活性差异。于 20 只裸鼠背部左、右侧各取 1 处,分别注射两组脂肪至皮下,每处注射 400 mg 脂肪。注射后 4、12 周,观察注射区反应后,取出移植物行大体观察、残留质量测量,组织学观察脂肪细胞,免疫组织化学染色观测微血管密度(microvessel density,MVD)。结果扫描电镜观察示,与对照组相比,实验组脂肪细胞饱满均一、脂肪血管结构更丰富;实验组葡萄糖转移量为(3.049±0.266)mmol/L,明显高于对照组的(2.668±0.250)mmol/L(t=2.956,P=0.010)。裸鼠体内注射后 4 周,仅对照组 1 处注射区发生脂肪液化;注射后 4、12 周,与对照组相比,实验组脂肪细胞形态更清晰、血管更丰富、坏死更少;实验组移植物残留质量、MVD 均高于对照组,比较差异有统计学意义(P<0.05)。结论新型前置式脂肪抽吸针能减少抽吸过程中对脂肪细胞的损害,进而提高移植脂肪成活率。

ObjectiveTo investigate the effect of a new front opening liposuction cannula on the survival of transplanted fat tissue.MethodsTwo groups of fats were obtained from the left and right sides of a female patient during the abdomen liposuction surgery, respectively. And the fats in experimental group and control group were harvested by the new front opening liposuction cannula and the side hole liposuction cannula, respectively. The differences of adipocyte activity in vitro between 2 groups were compared by observation under the electron scanning microscopy and the glucose transportation test. Then, the fats in 2 groups (n=20) were injected subcutaneously into the back of 20 nude mice (400 mg fats per injection zone). The differences of the injection area reactions, remaining weight, histological characteristics, and microvessel density (MVD) between 2 groups were compared after 4 weeks and 12 weeks.ResultsCompared with the control group, in vitro, the adipocytes were more plump and the vascular structures were more abundant. The glucose transportation quantities were (3.049±0.266) mmol/L and (2.668±0.250) mmol/L in experimental and control groups, showing significant difference between groups (t=2.956, P=0.010). There was only one fat liquefaction occurred in the injection zone of the control group after 4 weeks. The experimental group had more clear adipocytes, more vessels, and less inflammation and necrosis than the control group. The remaining weight and MVD were higher in the experimental group than in the control group after 4 and 12 weeks, showing significant differences (P<0.05).ConclusionThe new front opening liposuction cannula can reduce the damage of adipocytes and improve the survival of transplanted fat tissue.

关键词: 脂肪移植; 新型前置式脂肪抽吸针; 脂肪细胞活性

Key words: Fat transplantation; new front opening liposuction cannula; adipocyte viability

引用本文: 谈希, 雷肖璇, 郑志芳, 程飚, 邹彦龙, 王红霞, 王珍祥. 新型前置式脂肪抽吸针对移植脂肪成活影响的实验研究. 中国修复重建外科杂志, 2018, 32(3): 363-368. doi: 10.7507/1002-1892.201711026 复制

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